Single-particle cryo-electron microscopy (cryo-EM) has emerged as a powerful means in modelling macromolecular structures at near-atomic resolution (3–5 Å) 1. While high-resolution density maps enable ...

Cryo-electron microscopy (cryo-EM) has become a leading approach for protein structure determination, but it remains challenging to accurately model atomic structures with cryo-EM density maps. We ...