Given the association between HDR gene mutation and mammary tumour predisposition, we first tested this I-SceI DR-GFP system in mammary cells. Epithelial cells were isolated from the fourth inguinal ...

In recent years, CRISPR-Cas9 genome editing has enjoyed spectacular success because it combines flexibility and usability. The Cas9 nuclease need only be loaded with a guide RNA to direct it to almost ...

DURHAM, N.C., October 24, 2024--(BUSINESS WIRE)--Precision BioSciences, Inc. (Nasdaq: DTIL), a clinical stage gene editing company utilizing its novel proprietary ARCUS® platform to develop in vivo ...

CRISPR-Cas9 has increased the accessibility of genome engineering due to its ease of use and ability to cause double strand breaks (DSBs) at almost any locus of interest. DSBs are repaired in cells by ...

The CRISPR-Cas9 system derived from Streptococcus pyogenes uses the Cas9 nuclease protein that complexes with a tracrRNA and a targeting crRNA containing a 20 nucleotide guide sequence complementary ...

CORALVILLE, Iowa--(BUSINESS WIRE)--#ALTRHDRDonorBlocks--Global genomics solutions provider Integrated DNA Technologies (IDT) is empowering researchers to drive scientific discoveries in gene editing ...

Six scientists question the authors’ conclusions about the DNA repair process that led to the human embryos’ correction of a defective gene. In this webinar, Evelien Van Hamme and Elina Kuznecova will ...

The precise insertion of DNA sequences using the homology-directed repair (HDR) pathway is one potential use of the CRISPR/Cas genome editing technology. Various circumstances could influence the ...

DURHAM, N.C.--(BUSINESS WIRE)--Precision BioSciences, Inc. (Nasdaq: DTIL), a clinical stage gene editing company utilizing its novel proprietary ARCUS® platform to develop in vivo gene editing ...

Basics of homology-directed repair (HDR) using CRISPR-Cas9 Selection of CRISPR RNAs (crRNAs) for HDR Recommendations for design of a synthetic oligo or plasmid donor template Application of endogenous ...